2-D Proteome Analysis Protocols
A step-by-step tour through the complete process of doing proteomics. With easy-to-follow instructions, complete with many helpful hints and explanations, leading investigators and pioneers in the field show how to make protein extracts, reproducibly run them on 2-D gels, detect them, analyze the data, and precisely identify each protein. The book covers the latest methods of using carrier ampholytes in the 1st dimension, casting and running immobilized pH gradient 2-D gels, MALDI-TOF-based peptide mapping, automated tandem mass spectrometry, and nanoelectrospray ionization technology. For the 2nd dimension, there are methods for running flat…
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Produktdetails
- ISBN: 978-1-61737-060-1
- EAN: 9781617370601
- Produktnummer: 10768166
- Verlag: Springer Nature
- Sprache: Englisch
- Erscheinungsjahr: 2010
- Seitenangabe: 601 S.
- Masse: H22.9 cm x B15.2 cm x D3.5 cm 898 g
- Auflage: Softcover of Or
- Reihenbandnummer: 112
- Gewicht: 898
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