Engineering Complex Phenotypes in Industrial Strains

Sets forth current strategies for engineering biocatalysts for the production of bulk chemicals and biofuels With coverage of biocatalysts ranging from E. coli and Streptomyces to yeast and microalgae, this book explains current trends and developments in engineering industrial strains for the production of bulk chemicals and biofuels from renewable biomass. The authors guide readers through tested and proven tools and methodologies, providing plenty of examples that enable readers to engineer the complex traits and phenotypes needed for particular biocatalysts and bioprocesses. Engineering Complex Phenotypes in Industrial Strains features contributions from pioneers and leading international experts in the field. These authors offer insights into their thought processes as they initiate and develop a commercial biocatalyst engineering project. The first five chapters describe the current tools and technologies available for engineering and characterizing a complex phenotype in an industrial strain. The next four chapters feature detailed case studies: Clavulanic acid strain improvement program Metabolic engineering of recombinant E. coli for the production of 3-hydroxypropionate Complex system engineering: case study for an unsequenced microalga Meiotic recombination-based genome shuffling of Saccharomyces cerevisiae and Scheffersomyces stipitis for increased inhibitor tolerance to lignocellulosic substrate toxicity These case studies underscore the many disciplines that underlie the engineering of biocatalysts as well as illustrate in detail the application of the tools and methodologies discussed in the first half of the book. Engineering Complex Phenotypes in Industrial Strains is ideal for students and professionals in chemical engineering who need to understand the science and practice of engineering biocatalysts for a broad range of industrial applications.