Principles and Reactions of Protein Extraction, Purification, and Characterization

This book describes the principles and reactions for most techniques of protein extraction, purification, and characterization. The second edition covers modern techniques that are extensively used in protein identification and analyses including NMR, mass spectrometry, recombinant protein, and database search. It incorporates NMR and mass spectrometry for protein identification, use of computers and websites for protein annotation, and identification of commonly modified proteins. The text also expands coverage of the recombinant proteins. Two new chapters address the identification and annotation of the target protein and the identification of other posttranslational modifications. Key selling features:Features new chapters on the identification and annotation of the target protein and the identification of other posttranslational modificationsIncorporates NMR and mass spectrometry for protein identification, use of computers and web sites for protein annotation, and identification of commonly modified proteinsExpands coverage of the recombinant proteins (preparation, extraction, and purification including His-tagged columns)Describes the principles and reactions for most techniques of protein extraction, purification, and characterizationProvides background knowledge on most protein techniques with advantages and disadvantages and useful advice on their applications