Culture of Human Tumor Cells

Human cell culture in experimental cancer research is taking on new importance in the post-genomic era, as new methods for genome-wide analysis allow researchers to analyze the effects of altered genomes on the cell. Roswitha Pfragner and R. Ian Freshney's critical compilation introduces culture of human tumor cells, from basic principles to detailed protocols, to the seasoned researcher and novice alike. Chapters are organized by tumors of clinical significance, and offer informative overviews of pathology, preparation, troubleshooting, applications, alternative methods, and a list of suppliers. This wide-ranging text covers culture for lung tumors, gastric cancer, colorectal cancer, pancreas, bladder, prostate, ovary, cervix, mammary carcinoma, myoepithelium, squamous, melanoma, lymphoma, glioma, and neuroendocrine tumors. In addition, Culture of Human Tumor Cells: Provides stepwise protocols by recognized experts Presents detailed techniques for initiation, propagation, and characterization of tumor cell lines Includes extensive listing of vendors for equipment and other cell culture products Discusses drug treatment, selection, differentiation, assays for malignant cells, risks, and applications Offers safety precautions, helpful tips, and a list of abbreviations Provides specific, practical details on how to prepare media and reagents for the isolation of culture protocols Scientists, research clinicians, students, and technicians in pathology, cancer biology, cell biology, biochemistry, biotechnology, and medicine will find Culture of Human Tumor Cells to be an incomparable resource.