Guide to Electroporation and Electrofusion

Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques.Covers fundamentals of electroporation and electrofusion in detail: Molecular events, Mechanisms, Kinetics, Gives extensive practical information, The latest applications, Controlling parameters to maximize efficiency, Available instrumentationPresents applications of electroporation and electrofusion in current research situationsState-of-the-art modifications to electrical pulses and generatorsApplication of electroporation and electrofusion to unique, alternative cell and tissue typesGives straightforward, detailed, easy-to-follow protocols for Formation of human hybridomasIntroduction of genetic material into plant cells and pollenTransfection of mammalian cellsTransformation of bacteria, plants, and yeastProduction of altered embryosOptimization of electroporation by using reporter genesComprehensive and up-to-dateConvenient bench-top formatApproximately 125 illustrations complement the textComplete references with article titlesWritten by leading authorities in electroporation and electrofusion