Antidote properties of Emblica officinalis

Amla seeds are reported to have higher therapeutic potential in comparison to the fruits in which they reside. Thus, the aim of the present study was to evaluate the in-vitro antioxidant, anti-inflammatory, and anti-microbial effect of seed extracts on Gacrtn Emblica Officinalis (Amla). Amla has been used for thousands of years to conserve food health and treat diseases. The bioactive from the seeds were fractionated sequentially with hexane, chloroform, diethyl ether, and methanol solvents, in the increasing order of polarity. Total phenolic and flavonoid contents were estimated. These extracts were assessed for the antioxidant property by DPPH (1,1-diphenyl-2-picryl-hydrazyl) Method and reducing power assay was carried out using the FeCl3 method. Inhibition of 15-lipoxygenase (LO) by these extracts at 5 - 25µg to assess the anti-inflammatory capacity was studied. Antibacterial activity against some human pathogenic bacteria was tested by the agar disc diffusion method. Among all the organic solvent extracts methanolic extracts exhibited good antioxidant and antibacterial activity. Methanolic extract with an IC50 value of 58 ugs for LOX inhibition is reported.